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[PDF] Top 20 Development of a Loop-Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples

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Development of a Loop-Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples

Development of a Loop-Mediated Isothermal Amplification Method for Detection of Histoplasma capsulatum DNA in Clinical Samples

... Fungal DNA extraction. Genomic DNA was extracted using a Qiagen DNeasy tissue kit (Qiagen, Valencia, CA), with several modifications to the manufacturer’s ...portion of the fungal mat was transferred ... See full document

7

Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense.

Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense.

... trypanosome DNA samples used in this study are shown in Table ...The samples which most had been passaged in mice were chosen to ensure a wide geographical representation, ... See full document

8

Rapid and sensitive detection of Plesiomonas shigelloides by loop-mediated isothermal amplification of the hugA gene.

Rapid and sensitive detection of Plesiomonas shigelloides by loop-mediated isothermal amplification of the hugA gene.

... positive amplification of P. shigelloides DNA was ...the detection limit of LAMP in human stool, serial 10-fold dilutions of a mid-log-phase culture of ...grown ... See full document

6

Rapid, simple and sensitive detection of Q fever by loop-mediated isothermal amplification of the htpAB gene.

Rapid, simple and sensitive detection of Q fever by loop-mediated isothermal amplification of the htpAB gene.

... pairs of blood samples from the acute phase and convalescence of patients with clinical assessment Q fever from Yi Li Prefecture, Xinjiang Province, in ...2008. Of these 24 ... See full document

6

Rapid and Sensitive Detection of the Main Contaminating Fungus Penicillium restrictum in Jet Fuel using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick

Rapid and Sensitive Detection of the Main Contaminating Fungus Penicillium restrictum in Jet Fuel using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick

... identification of microbial contamination based on biology and morphology require the cultivation of microorganisms [5, 8, ...1% of microbes that have been identified can actually be cultivated ... See full document

12

A loop-mediated isothermal amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: a diagnostic approach in a murine model.

A loop-mediated isothermal amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: a diagnostic approach in a murine model.

... limitation of these methods is their lack in sensitivity, especially in low-grade infections, as occurs in areas of low prevalence or in individuals with recent infections ... See full document

11

Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification

Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification

... A frican swine fever virus (ASFV) is an enveloped, complex, icosahedral virus with a double-stranded DNA genome of 170 to 190 kb in length (Takamatsu et al., 2013). It has been classified as the sole ... See full document

7

Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by loop-mediated isothermal amplification (LAMP).

Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by loop-mediated isothermal amplification (LAMP).

... genomic DNA at very high concentrations (i.e. .1 ng DNA/ m L equivalent to ...fg DNA/ m L equivalent to 10 2 to 10 23 parasites/ m ...as DNA from clinical samples or spiked blood ... See full document

10

Sensitive Detection of Plasmodium vivax Using a High-Throughput, Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) Platform: A Potential Novel Tool for Malaria Elimination.

Sensitive Detection of Plasmodium vivax Using a High-Throughput, Colourimetric Loop Mediated Isothermal Amplification (HtLAMP) Platform: A Potential Novel Tool for Malaria Elimination.

... specificity of the P. vivax HtLAMP was tested retrospectively on clinical samples from patients enrolled in a randomised controlled trial and case-control study per- formed in Sabah, ... See full document

16

Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method

Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method

... An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evalu- ated for sensitivity and specificity in detecting the presence of Salmonella ... See full document

7

Detection of head-to-tail DNA sequences of human bocavirus in clinical samples.

Detection of head-to-tail DNA sequences of human bocavirus in clinical samples.

... kind of possible concatemeric sequence postulated for the parvoviral replication cycle (figure ...listed in table ...cross-section of published HBoV sequences including the both reference sequences ... See full document

9

Fabrication Of Gold Nanoparticles-Modified Glassy Carbon Electrode And Its Application For Voltammetric Detection Of CrIII

Fabrication Of Gold Nanoparticles-Modified Glassy Carbon Electrode And Its Application For Voltammetric Detection Of CrIII

... mainly in Cr(III) and Cr(VI) oxidation states in aquatic ...found in the environment due to erosion of chromium-containing rocks, precipitation, terrestrial run-off and can be introduced by ... See full document

5

Application of time-series analysis for prediction of molding sand properties in production cycle

Application of time-series analysis for prediction of molding sand properties in production cycle

... nature of manufacturing processes and permits prediction of future values of the process parameters or production results on the basis of the past data, recorded in regular ...problems ... See full document

5

Comparison of the efficacies of loop-mediated isothermal amplification, fluorescence smear microscopy and culture for the diagnosis of tuberculosis.

Comparison of the efficacies of loop-mediated isothermal amplification, fluorescence smear microscopy and culture for the diagnosis of tuberculosis.

... microscopy in comparison with culture as the gold ...testing in series and parallel were also compared against the culture ...compared in series (LAMP performed only if smear positive and considered ... See full document

5

In Vitro Effect of Sulfamethoxazole-Trimethoprim against Histoplasma capsulatum var. capsulatum

In Vitro Effect of Sulfamethoxazole-Trimethoprim against Histoplasma capsulatum var. capsulatum

... effect of sulfamethox- azole or SMX-TMP against other pathogenic fungi, such as Cryptococcus neoformans (8) and Aspergillus fumigatus ...results of this study demonstrated for the first time the inhib- ... See full document

2

A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes.

A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes.

... antibodies in oral samples—however, the nucleic acid-based method presented here has additional potential ...analysis of accumulation of fluorescent signals [16]. In one ... See full document

11

Detection Of Hepatitis B Virus DNA In Moroccan Patients With Epithelial Ovarian Carcinoma EOC By Polymerase Chain Reaction

Detection Of Hepatitis B Virus DNA In Moroccan Patients With Epithelial Ovarian Carcinoma EOC By Polymerase Chain Reaction

... occurred in both surgical and chemotherapeutic techniques, the epithelial ovarian cancer remains the leading cause of death from gynecological malignancy in the world with 5-year survival rates lower ... See full document

3

In vitro effect of Trimethoprim against Histoplasma capsulatum var. capsulatum

In vitro effect of Trimethoprim against Histoplasma capsulatum var. capsulatum

... effect of sulfamethox- azole or SMX-TMP against other pathogenic fungi, such as Cryptococcus neoformans (8) and Aspergillus fumigatus ...results of this study demonstrated for the first time the inhib- ... See full document

2

The application of optical measurements for the determination of accuracy of gear wheels casts manufactured in the RT/RP process

The application of optical measurements for the determination of accuracy of gear wheels casts manufactured in the RT/RP process

... used in the production of casting ...spectrum of rapid prototyping uses can be expanded by the application of the rapid tooling ...One of the RT techniques is the direct manufacture ... See full document

4

An empirical approach for quantifying loop-mediated isothermal amplification (LAMP) using Escherichia coli as a model system.

An empirical approach for quantifying loop-mediated isothermal amplification (LAMP) using Escherichia coli as a model system.

... using loop mediated isothermal amplifica- tion (LAMP) is growing in popularity because of its practicality, speed and usefulness in laboratories and clinical settings ... See full document

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